Laboratory Rotation III

Objectives

This curricular unit aims to prepare the students for the laboratory work of their PhD thesis in research areas in aging and chronic diseases. Moreover, it aims to give students training in techniques and methods with which they are not familiar yet. It is expected that the students acquire laboratory skills, such as planning an experiment, performing it and analyzing the results. It is also expected that the students learn how to interpret the results and find the reasons behind failures and problems, as well as strategies to overcome these. At the end, the students should have acquired knowledge about specific techniques and most of all the general knowledge of what it means starting from a scientific question, finding strategies to answer it and interpret the meaning of the experimental data. Another important goal is to endow the students with oral and written communication skills and the discussion of the work with an audience.

General characterization

Code

1071003

Credits

10

Responsible teacher

Margarida Correia-Neves

Hours

Weekly - Available soon

Total - Available soon

Teaching language

Portuguese/English

Prerequisites

 

Bibliography

Molecular cloning : a laboratory manual / Joseph Sambrook, David W. Russell, Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory.

Current protocols in Cell Biology, Cell Culture, Imaging and Microscopy, Molecular Biology and Supporting Lab Techniques, John Wiley & Sons

International journals of reference such as Nature, Science, Cell, PNAS

Teaching method

Daily, the supervisor will discuss the experimental plan and the student's questions answered. The student is expected to understand why the experimental strategy is the most adequate to answer the initial question. The detailed protocol will also be reviewed with the student. When the experiment ends, or at least weekly, the student and the supervisor will discuss the results obtained and possible interpretations, deciding together what is the most logical next step.

Evaluation method

The evaluation will be based on a written report that should be handed in a pre-established date, the oral presentation of this report and finally the evaluation of the supervisor. Each one of these will weight 1/3 of the final score.

Subject matter

- Cytokine regulation of the innate immune response to chronic bacterial, viral and fungal infections;

- Mechanisms and alterations of the acquired immune response to bacterial, fungal and viral infections;

- Novel therapies for chronic mycobacterial infections;

- Chronic alterations of the immune system in patients with HIV infections;

- Molecular and cellular mechanisms responsible for the immunosenescence of the immune system in aging;

- Autophagy and apoptosis in the context of chronic diseases

- The interaction between the nervous system and the immune system in aging and chronic diseases;

- The effects of stress in adults and early life the Central Nervous System and other organic systems;

- The cellular and molecular mechanisms responsible for chronic diseases of the central nervous system and pain;

- Inflammation, obesity and other metabolic disorders;

- Novel surgical methods to treat chronic and developmental diseases.

Programs

Programs where the course is taught: