Human Cytomegalovirus Infections
1. Knowledge of CMV infections. Application of different diagnostic techniques depending on the host and clinical context (immunocompetent, immunocompromised, pregnant, newborn);
2. Knowledge of the application of real time PCR to the diagnosis of cytomegalic infection;
3. Contact with the diagnostic methods used for CMV (Culture; Antigenemia; Molecular Biology Methods: PCR "in-house technique"; Real-time PCR; Serology: routine immunoassay assays, confirmatory tests - Western blot, IgG avidity);
4. Recognition of the importance of genotypic detection of ganciclovir resistance. Knowledge of the different steps of this detection.
Weekly - 3
Total - 38
- Paulo Paixão, Sofia Almeida, Paula A. Videira, Dário Ligeiro, Teresa Marques. “Screening of congenital cytomegalovirus infection by a real time PCR in urine pools”. European Journal of Pediatrics, 2012; 171 (1): 125129.
- Kotton CN, Kumar D, Caliendo AM, Asberg A, Chou S, DanzigerIsakov L, Humar A. Updated international consensus guidelines on the management of cytomegalovirus in solidorgan transplantation. Transplantation. 2013;96(4):33360.
- Revello MG, Gerna G. Pathogenesis and prenatal diagnosis of human cytomegalovirus infection. J Clin Virol. 2004;29(2):7183.
Student assessment – Written examination finally multiple choice test of 20 questions (duration 30 minutes) 50%.
Oral presentation and discussion of topics by groups of two students. Themes are distributed in 1st class and the presentation of each theme has a duration of 20 minutes 50%.
Themes and Contents of the lecture (s):
- Introduction to the diagnosis of CMV infections. Application of different techniques depending on the host and the clinical context (immunocompetent, immunocompromised, pregnant, newborn);
- Antivirals acting on CMV. Resistance and its detection;
- Real time PCR (general concepts and specific application to CMV);
- Discussion of clinical and laboratory cases;
- Discussion of the results obtained in practical teaching;
Themes and Contents of the practical class (s):
- Culture: observation of flasks with characteristic cytopathogenic effects; preparation of tubes for shell-vial culture, inoculation, development and observation after incubation;
- Antigenemia: separation and preparation of slides; staining and observation. PCR (in-house technique): extraction and amplification PCR: detection of amplifiers;
- Serology: routine immunoassay assays, confirmatory tests (Western blot, IgG avidity);
- PCR (in-house technique): extraction and amplification; detection of amplifiers;
- Genotypic detection of ganciclovir resistance: extraction, amplification and observation of sequences;
- Real time PCR: extraction, amplification, detection.
Programs where the course is taught: