Mechanisms of Antibiotic Resistance


1. To know the main examples of antibiotic resistant bacteria in the hospital and in the community. To be able to interpret and describe the associated resistance mechanisms;

2. To understand the intracellular action of antibiotics;

3. To understand the importance ofbiocides and the formation of bacterial biofilms on the development of resistance to antimicrobials;

4. To have a global perspective on the impact of antibiotics on the environment according to the “One Health” concept;

5. To be able to interpret data on antibiotic susceptibility laboratorial tests;

6. To be able to design and perform independently experimental protocols to determine the mechanism(s) of resistance to a given antibiotic.

General characterization





Responsible teacher

Isabel Maria dos Santos Leitão Couto


Weekly - 3

Total - 50

Teaching language



Available soon


Available soon

Teaching method

The theoretical concepts are taught by an expositive method, promoting interaction with students. The laboratory classes are carried out in groups (2-4 students/group) in which each group will receive several bacterial strains for which it shall determine the phenotype (susceptible/resistant) towards several antibiotics; design and execute several experiments to determine the possible mechanisms behind specific resistance phenotypes, using the information provided on the theoretical classes and supporting bibliography.

Evaluation method

Students will be assessed through:

i) a written exam of multiple choice questions, focusing on the content of theoretical and laboratory classes (60% total classification)

ii) by the preparation, presentation and discussion of the practical case-study (40% total classification).

Both components are rated in a 0 to 20 values scale; students rated 9.5 or higher in both components (theoretical exam and case-study presentation) will be approved.

Subject matter

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Programs where the course is taught: