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Enzyme Technology

Objectives

The students should acquire knowledge to understand the processes of selection, production and purification of a biocatalyst as well as its application and modelling in bioreactors, namely through immobilization techniques.

General characterization

Code

10688

Credits

6.0

Responsible teacher

Ana Cecília Afonso Roque, Maria Filomena Andrade de Freitas

Hours

Weekly - 5

Total - 68

Teaching language

Português

Prerequisites

Available soon

Bibliography

[1] Klaus Buchholz, Volker Kasche, Uwe Theo Bornscheuer (2005), “Biocatalysts and Enzyme Technology”, Wiley-VCH.

[2] Gama, M, Aires-Barros, MR, Cabral, J (2003), "Engenharia Enzinmática", Lidel, ISBN 9789727572724 | 272 págs.

Teaching method

The unit of Enzyme Technology consists of three components: theoretical (T), theory and practice (TP) and practice (P).

The TP component is based on problem solving with students about the content covered in the theoretical component.

Evaluation method

Evaluation of Enzyme Technology unit will perform as follows for students of first enrollment :

Individual assessment component T and TP , worth 60 % of final grade : Two tests to be done during the class period OR an exam in exam period (average of 2 tests OR endnote examination will have to be at least 10 val . ) . If the student does not obtain 10val . the average of the two tests will have to sit the examination .

Evaluation of P component , worth 40 % of final grade : Two laboratory classes frequency MANDATORY , which are elaborated on report that will be discussed with teachers . The discussion with teachers will last 15min/grupo . The delivery and discussion of the reports is also mandatory . The student must obtain at least 10 marks in the report + discussion of each of the reports .

Evaluation of Enzyme Technology unit will perform the following to students from Monday ( OR more) description :

Individual assessment component T and TP , worth 60 % of final grade :: Two tests to be done during the class period OR an exam in exam period (average of 2 tests OR endnote examination will have to be at least 10 val . .) If the student does not obtain 10val . the average of the two tests will have to sit the examination .

Evaluation of component P : If the student has had the attendance practice in previous years and got a final this component greater than or equal to 10 val . NOT need to perform the P component this academic year . The score at the P component in previous years remain in effect this school year worth 40 % of the final grade . However , if the student wishes to improve the grade obtained in previous years can do it , and to this warn the teacher that intention DURING THE 1st WEEK OF CLASSES .

Subject matter

(i) Enzyme structure and function.

(ii) Activity and stability of enzymes.

(iii) Enzyme production and purification.Sources of biocatalysts. Methods for enzyme recovery: solid-liquid separation, cell lysis and disruption, protein precipitation, aqueous two-phase liquid extraction, chromatography.

(iv) Protein engineering, molecular modelling. Immobilization of biocatalysts.

(v) Characterization of immobilised biocatalysts. Effects of immobilization on enzyme kinetics, internal and external diffusional limitations.

(vi) Reactors with biocatalysts (soluble and immobilized biocatalysts), project and analysis. Continuous stirred tank reactor, plug flow reactor, batch reactor.