General Biochemistry A
After the course it is expected that the students know the basic structural features of the major biological macromolecules and understand the physical-chemical principles underlying the relationship between structure and function. The students should also understand the strategy, regulation, and bioenergetics of the central metabolic pathway. It is expected that the students apply the concepts developed in the lectures to the analysis of experimental results obtained in the laboratory classes and to problem solving.
Maria Alice Santos Pereira
Weekly - 4
Total - 64
Strongly recommended a basic background of organic chemistry and general chemistry.
LEHNINGER PRINCIPLES OF BIOCHEMISTRY
Nelson, D.L., & Cox, M.M.
W.H. Freeman and Company, San Francisco, 7th ed., 2017
FUNDAMENTALS OF BIOCHEMISTRY. LIFE AT THE MOLECULAR LEVEL
Voet, D., Voet, J.G. & Pratt, C.W.
John Wiley & Sons, Inc., New York, 5th ed., 2016
Berg, J.M., Tymoczko, J.L., & Stryer L.
W. H. Freeman and Company, San Francisco. 8th ed., 2015
BIOCHEMISTRY. A SHORT COURSE
Berg, J.M., Tymoczko, J.L., & Stryer L.
W. H. Freeman and Company, San Francisco. 3rd ed, 2015
The course is organized in lectures, laboratory classes and workshops. The students must apply the concepts developed in the lectures to the analysis of the experimental results obtained in the laboratory and to problem solving in the workshops.
The final grade will be calculated according to the following formula:
Final grade = 0.35 x Grade Questionnaires + 0.65 Grade Individual tests
Students will pass the discipline with a grade greater than or equal to 9.5 points.
Mandatory frequency of the 5 practical sessions (3 laboratory and 2 online)
The punctuality of the student, the preparation of practical classes, the execution of experimental work, will contribute to obtaining the frequency
II. Evaluation of the component T
1. Continuous distance assessment
- 5 questionnaires (Q1 to Q5) for group resolution; eliminating the lowest grade.
- 2 individual online tests (Test1 and Test2). Students will pass with a mean grade of the 2 tests greater than or equal to 8.0 points.
The questionnaires will have a duration of 20 to 30 min and the individual tests 60 min.
2. Evaluation by final exam
- The marks of all elements of evaluation will be rounded up to the decimal; the grades of each component will be included in the final classification formula, also rounded to the decimal.
- Students who have not passed continuous assessment are admitted to the final exam. To be successful, the classification in this exam must be equal to or greater than 9.5.
- Improvement of the final classification is made in the final exam and only for the T component.
In any case, the Responsible of the UC reserves the right to make an oral exam, for final approval, to any student enrolled in the discipline.
4. Summary of the lectures:
1- Introduction to Biochemistry (Biological structures, metabolic pathways, expression and transmission of genetic information).
2- The amino acids properties: peptide bonds - Classification and characteristics. Acid-base properties
3- Levels of protein structure organization: primary, secondary (alpha helices, beta sheets and other structural elements), tertiary and quaternary structures. Protein isolation, purification and sequencing.
4- Fibrous proteins (Keratin, silk, collagen) and globular protein (Myoglobin and hemoglobin)
5- Structure-function mechanisms in the hemoglobin.
6- Enzyme kinetics Inhibition and regulation.
7- Enzyme mechanisms: Lisozima, Ribonuclease, Carboxipeptidase, Quimotripsina.
8- Carbohydrates (Classification and function). Monosaccharides and structural and storage polysaccharides (cellulose, chitin, starch and glycogen). Glycoproteins (Proteoglycans and glycosylated proteins).
9- Biological Membranes: structure and dynamic. Biological transport: Passive-mediated transport, active-transport. Ion channels, Uniport, symport, and antiporte systems.
10- Nucleic acids (RNA and DNA): structure and function. Denaturation and Renaturation of nucleic acid. Restriction endonucleases: the chain- terminator method of DNA sequencing and Manual sequencing. Recombinant DNA technology. PCR and site-directed mutagenesis techniques.
11- Metabolism (Catabolism and anabolism) and Bioenergetics: Glycolysis, lactic and alcoholic fermentation, Citric acid Cycle, electron transport chain and oxidative phosphorylation.
12 – Photosynthesis.
1 -Amino acid and Proteins – Acid-base properties
2 - Structure polypeptides and proteins.
3 - Enzyme kinetics
4 - Nucleic acids. Biological membranes.
5 – Metabolism
Laboratory sessions :
1 – Amino acid titrations
2 – Separation of biological compounds by chromatography
A – Gel filtration
B – Ionic Chromatography
3 – Enzymatic characterization of Fusarium graminearum beta-glucosidas
4 - Protein determination
Programs where the course is taught: