Entomological Techniques in Epidemiological Evaluation
After this unit, students should be able to: 1. Define the objectives of a culicideological survey, respective field work directed to mosquito collection; 2. Select mosquito capture methods (adult and immature forms); 3. Choose preservation methods for the transport of specimens to the laboratory; 4. Plan and prepare materials for field work; 5. Carry out a field work, and the selected mosquito collection methods; 6. Process the captured specimens for their identification; 7. Identify morphologically mosquito specimens; 8.P rocess the specimens in agreement with the various objectives of the survey; 9. Organize, process and analyse the results obtained; 10. Discuss critically the results obtained, methods used, and propose adaptations if due.
Weekly - Se a UC for oferecida como opcional, o horário será disponibilizado no 2º semestre
Total - 30,5
• Wernsdorfer W.H. and McGregor, (1988). Malaria, principles and practice of Malariology. Churchill Livingstone Inc., New York, USA. • Reiter, P. and Gubler, D. J. (1997). Surveillance and control of urban dengue vectors. Dengue and Dengue Hemorrhagic Fever (Gubler, D.J. and Kuno, G. eds., pp. 425462). CAB International, Wallingford, UK. • Ribeiro, H. and Ramos, H.C. (1999). Identification keys of the mosquitoes (Diptera:Culicidae) of Continental Portugal, Açores and Madeira. European Mosquito Bulletin 3: 111. • Service, M.W. (1993). Mosquitoes (Culicidae) in Medical Insects and Arachnids. (Lane, R.P. and Crosskey, R.W. eds., pp. 120240) Chapman and Hall, UK. • Service, M.W. (1999). Mosquito Ecology: Field sampling methods. Chapman and Hall, London, UK.
Lectures; Theoretical-practical training classes; Laboratorial practical sessions; Tutorial supervision; Seminar; Autonomous work.
1. Continuous evaluation, based on daily presence and active participation in classes and field work 30%. 2. Seminar 30%. 3. Written report, with circa 2000 words (except graphs and reference list) 40%.
I. Culicideological survey: objectives, planning of respective field work directed to mosquito collection; II. Selection of mosquito capture methods (adult and immature forms) according to the objectives of the survey. Advantages, disadvantages and limitations; III. Methods of specimen preservation for the transport to the laboratory, according to survey objectives; IV. Planning and preparation of materials for field work; V. Conduction of field work, and execution of selected mosquito collection methods; VI. Processing of captured specimens for their identification; VII. Morphological identification of mosquito specimens; VIII. Processing collected specimens according to the various objectives of the survey; IX. Organization of data from field and lab work, construction of a data base, and statistical analysis; X. Critical analysis and discussion of the results obtained, methods used, and proposal of adaptations if due.
Programs where the course is taught: