Tissue Engineering
Objectives
Excesso de caracteres – Máximo 1000.
At the end of this course the student will have acquired knowledge, skills and competencies that allow him to design and write a research project in the areas of tissue engineering and in vitro models (organoids) for disease studies and evaluation of drugs and cosmetics.
For this, the student will have acquired knowledge about:
- biomaterials - polymers and ceramics of natural or synthetic origin;
- scaffold production techniques;
- types of cells (stem and adult) used in the construction of organoids and substitutes;
- the problem of the vascularization of organoids and organ / tissue substitutes
- models and skin substitutes for Regenerative Medicine and evaluation of cosmetics.
- development of organoid as models for the study of diseases
The student will also have acquired skills:
- in the production of 3D structures for iVM and TE;
- in the physical-chemical characterization of scaffolds;
- in the culture of animal cells;
- in seeding and analysis of cell cultures in biomaterials.
General characterization
Code
12486
Credits
6.0
Responsible teacher
Jorge Alexandre Monteiro de Carvalho e Silva
Hours
Weekly - 4
Total - 56
Teaching language
Inglês
Prerequisites
None
Bibliography
Main Bibliography:
- Clemens A. van Blitterswijk and Jan de Boer (editors), Tissue Engineering, Elsevier, 2015
As additional bibliography for specific parts of the program, students are provided with several review and research articles.
Teaching method
The teaching method followed is based on theoretical classes and practical laboratory classes. In the theoretical classes the subjects that comprise the syllabus are discussed through the discussion of concepts, methods and examples of laboratory and clinical studies. In practical classes, students produce scaffolds and perform their physical, chemical and biological characterization.
Evaluation method
The final grade is the weighted average of the marks obtained in the three components of the evaluation:
1- Practical work (30%): carried out in groups of 2 students and evaluated based on the reports prepared.
2- Seminar (30%): presentation and discussion, in groups of 2 students, on the state of the art of in vitro models for drug and cosmetic evaluation and Tissue Engineering of a tissue or organ of choice.
3- Research project (40%): individual work, consists in the elaboration of the scientific component of a research project in the area of the unit according to the FCT/MCTES rules.
Subject matter
Biomaterials for IVM and TE
The extracellular Matrix as a Bioscaffold for IVM and TE
Scaffold Design and Fabrication
Cell Culture
Stem Cells for IVM and TE
Controlled Release Strategies in Tissue Engineering
Bioreactors: Enabling Technologies for Research and Manufacturing
Vascularization, Survival, and Functionality of Tissue-Engineered Constructs
Skin Engineering for Regenerative Medicine and Cosmetics testing
Tissue Engineering of Organ Systems
Organs-on-a-Chip
In vitro Disease Models
Labs:
Incorporation of silver nanoparticles in a PVP wound dressing
PCL aligned nanofibers for cell orientation
3D composite scaffolds for bone tissue engineering
Freeze-dried gelatin scaffolds for soft tissue engineering and organoid development