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  4. Biosystems Simulation for Life and Health Sciences
  5. Gene Expression Modulation

Gene Expression Modulation

Objectives

At the end of this curricular unit, the student will have acquired knowledge, skills and competences that allow them to:

-Understand the basic mechanisms of gene expression modulation.

-Be able to distinguish the main differences in the regulation of gene expression in Eukaryotes and Prokaryotes

-Know the molecular actors involved in the initiation of transcription in both types of cells

-Understanding the role of chromatin in the modulation of gene expression

-Know the main mechanisms of post-transcriptional regulation

-Know the main experimental techniques for studying the regulation of gene expression.

-Develop skills in the use of databases related to the modulation of gene expression. -Develop teamwork and communication skills

General characterization

Code

12497

Credits

6.0

Responsible teacher

Paula Maria Theriaga Mendes Bernardo Gonçalves

Hours

Weekly - 4

Total - 55

Teaching language

Inglês

Prerequisites

.

Bibliography

Lewin´s Genes XII

Review articles

Teaching method

In the theoretical part of the classes, the syllabus will be exposed in an interactive way.

In the practical part, students will practice using several databases containing information on the modulation of transcription.

In the process, they will also gather information for the preparation of n essay to be elaborated in groups.

Evaluation method

Tests during the semester (60%) andl essay (40%).

Subject matter

Topics covered include: 1) Basic mechanisms of gene expression: major differences between eukaryotes and prokaryotes 2) RNA polymerases and promoters in prokaryotes 3) Regulation of transcription in prokaryotes: activation, repression and attenuation 4) Mechanisms of gene expression regulation in eukaryotes 5) Expressed Quantitative Trait Loci (eQTLs) 6) Mechanisms of post-transcriptional regulation 7) Influence of chromatin structure and modifications on the regulation of gene expression 8) Regulatory RNAs In the practical sessions students are expected to prepare individual essays under supervision on a topic related to regulation of gene expression, using appropriate databases such as Ensembl, GTEX and miRanda.