Microbiology B


 To understand the diversity of microbial life and the relationships between the main groups of microorganisms in an evolutionary perspective, as well as metabolic and structural diversity. To acquire basic knowlege on molecular identification of microrganisms and of microbial ecology.

  To learn the basic laboratory techniques of microbiology: optical microscopy; microbial culture and aseptic technique; isolation of microorganisms.

General characterization





Responsible teacher

José Paulo Nunes de Sousa Sampaio, Paula Maria Theriaga Mendes Bernardo Gonçalves


Weekly - 4

Total - 27

Teaching language



Fundamentals of Biochemistry.


- Lecture handouts and other learning support items posted in the course Moodle webpage.

- M.T. Madigan et al., “Brock Biology of Microorganisms”, 12thed., Pearson, 2009 (or other editions of the same book).

Teaching method

Teaching strategy involves 1.5 hour lectures and 3 hour lab sessions.

Evaluation method

Available soon

Subject matter

Lectures: Unity and diversity of life on Earth; major groups of microorganisms and their importance. Microbial cell structure; prokaryotes: eubacteria and archaebacteria; comparison with microbial eukaryotes. Microbial nutrition and metabolism: diversity of biosynthetic (C & N) and bioenergetic processes and their classification; consequences of microbial metabolism. Classification and evolution of cellular organisms: historical perspective; current classification systems; molecular phylogenies; origin of the eukaryotic cell. Introductory Mycology: fungal classification; overview of morphological, physiological and metabolic characteristics; ecological and biotechnological impact of fungi. Introductory Bacteriology: bacterial taxonomy and major phylogenetic lineages of eubacteria and archaebacteria; overview of morphological, physiological and metabolic characteristics; ecological, clinical and biotechnological impact of bacteria.


Lab sessions: Basic microbiological techniques: preparation of culture media; sterilization; aseptic technique. Introduction to laboratory culture of microorganisms. Light microscopy: eukaryotic and prokaryotic cells. Microscopical examination of environmental samples (soil/water). Basic methods for the isolation of microorganisms:isolation of endospore-forming bacteria (Bacillus) from soil; isolation of yeasts from fermenting fruit.