The main objectives are:
1) learning the application of different chromatographic techniques;
2) learning the sample preparation techniques;
3) understanding the qualitative and quantitative analytical chemistry.
João Paulo da Costa Noronha
Weekly - 3
Total - 53
Basic knowledge in Chemistry and Organic Chemistry.
Basic knowledge of statistics.
The Troubleshooting and Maintenance Guide for Gas Chromatographers, Dean Rood, 4th Ed., Wiley-VCH, Weinheim, 2007.
Interpretation of Mass Spectra, McLafferty, F.W. and Turecek, F., University Science Books, Mill Valley, CA, 4th Edition, 1993.
Introduction to Protein Mass Spectrometry, Pradip K. Ghosh, Academic Press 2016, https://doi.org/10.1016/C2014-0-02130-2, ISBN: 978-0-12-802102-6
Organic Laboratory Techniques, Ralph J. Fessenden, Joan S. Fessenden and Patty Feist, Brooks/Cole (Ed.), 2001.
Principles of Analytical Chemistry – A Textbook, M. Valcárcel, Springer-Verlag (Ed.), 2000.
Quantitative Chemical Analysis, 8th Edition - Daniel C. Harris - W. H. Freeman (2010)
Statistics for Analytical Chemistry; J.C. Miller and J.N. Miller, Ellis Horwood Limited, Chichester, England, 1988.
Theoretical subjects are presented and explained to students in lectures (1h / week).
- Lectures taught using a "data-show", accompanied by supplementary bibliography previously available on the course page in CLIP/Moodle. In the first class of the semester all information about the curricular unit will be presented. Evaluation will be discussed as well.
Practical lectures, 2.5h/week in a working group.
It will be involved: GC, HPLC (LC-UV, LC-DAD), sample preparation methods (including classic methods, SPE and SPME). Introduction to multidimensional methods (GC-MS, LC-MS/MS)
- 2 theory tests (individual): 25% each
- 1 Report (article format) + Presentation (group): 25%
- 2 Paper presentation in Note format (group): 25%
In case of a negative weighted average final evaluation (less then 9.5 in 20), the grade corresponding to the theoretical component will have to be obtained by examination that in this case will be worth 50% of the final grade of the UC.
The frequency discipline is mandatory. Derives 70% attendance in lectures and attendance at all practical classe.
Instrumentation: HPLC, GC, GC/MS and LC-MS/MS - basic components and how to use.
Sample preparation: SPE, SPME, liquid extraction, lyophilization, dryness and derivatization.
Analytical method validation.
Programs where the course is taught: