Monoclonal Antibodies: Obtention and Applications


Acquire knowledge and concepts on how to:
1. Obtain monoclonal antibodies.
2. Obtain recombinant antibodies.
3. Apply to diagnosis and therapy.
4. Apply monoclonal antibogies for immunolocalization of important molecules in the biological activity of parasites.
5. Apply basic techniques applied to the characterization and purification of antibodies.
Acquire experimental skills in:
1. Animal Handling.
2. Immunization.
3. Culture of animal cells in vitro.
4. Collection and selection of hybridomes.
5. Immunocytochemistry and immunofluorescence techniques.

General characterization





Responsible teacher

Carlos Novo


Weekly - Se a UC for oferecida como opcional, o horário será disponibilizado no 2º semestre

Total - 44,5

Teaching language



Not applicable


• Galluzzi L, Vacchelli E, et al. Trial Watch: Monoclonal antibodies in cancer therapy. Oncoimmunology. 2012 Jan 1;1(1):2837.
• Galluzzi L, Vacchelli E, Fridman WH, Galon J, Sautès-Fridman C, Tartour E, Zucman-Rossi J, Zitvogel L, Kroemer G. Trial Watch: Monoclonal antibodies in cancer therapy. Oncoimmunology. 2012 Jan 1;1(1):2837.
• Altshuler EP, Serebryanaya DV, Katrukha AG. Generation of recombinant antibodies and means for increasing their affinity. Biochemistry (Mosc). 2010 Dec;75(13):1584605.
• Ramos-Vara JA. Technical aspects of immunohistochemistry. Vet Pathol. 2005 Jul;42(4):40526.
• Herzenberg LA, De Rosa SC. Monoclonal antibodies and the FACS: complementary tools for immunobiology and medicine. Immunol Today. 2000 Aug;21(8):38390.
• Stolf BS, Smyrnias I, Lopes LR, Vendramin A, Goto H, Laurindo FR, Shah AM, Santos CX. Protein disulfide isomerase and host-pathogen interaction. ScientificWorldJournal. 2011;11:174961. Epub 2011 Oct 18.

Teaching method

Lectures and theoretical-practical classes.

Evaluation method

Assessment is through the written analysis of an article chosen from two suggested to the students about topics covered in this CU.

Subject matter

I. Obtaining monoclonal antibodies.
II. Somatic recombination.
III. Basic structure of immunoglobulins. Terminology, structure-function relationships. Multicopy organization of the immunoglobulin coding genes. Somatic recombination of gene segments encoding immunoglobulin polymorphic regions.
VI. Recombinant antibodies: Preparation and applications.
V. Immunotherapy (Immunotherapy in hypersensitivity. Immunosuppression, applications in autoimmunity and transplantation. Immunotherapy in cancer).
VI. Flow cytometry and Luminex (Principles and applications.
VII. Structure of proteins and their analysis and prediction models.
VIII. Isomerases/chaperones and their Immunolocalization.
IX. Monoclonal antibodies in diagnostics of protozoa.
X. Mabs as diagnostic and therapeutic tools for parasites of veterinary interest.
XII. Antibodies as directional agents in hyperthermia.
XIII. Application to HIV diagnosis.
XIV. The monoclonal industry.


Programs where the course is taught: