Introduction to Molecular Genetics

Objectives

1. To understand the fundamental mechanisms underlying the dissemination and expression of genetic information.


2. To acknowledge and apply base stone methods in a genetics and molecular biology lab.


3. Understanding the main biotechnological applications of molecular genetics.

General characterization

Code

12592

Credits

6.0

Responsible teacher

Pedro Manuel Broa Costa, Pedro Miguel Ribeiro Viana Baptista

Hours

Weekly - 3

Total - 60

Teaching language

Português

Prerequisites

Preferable: Previous attendance to Cell and Molecular biology.

Bibliography

Miller, J.H. et al. Introduction to Genetic Analysis. W.H. Freeman & Company, NY, USA.

Pasternak, J.J. & Patten, C.L. Molecular Biotechnology. Principles and Applications of Recombinant DNA. ASM Press, Washington DC., USA. Lodish, H. et al. Molecular Cell

Biology. W.H. Freeman & Company, NY, USA. Scientific articles provided in classes.

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Scientific articles provided in classes.

Teaching method

The teaching methodology is based on the presentation of essential concepts applied to actual examples and problem solving. The acquisition of autonomous study is promoted, as well as in-class debate, thus combining expository with interpretive teaching. Classes are supported by digital traching material provided to students, namely slides and relevant scientific literature.

Evaluation method

Avaliação contínua

T: Final test [10 val., minimum required grade for frquency: 7 val.]

P: Group written assignment (from lab session results) + discussion [5 val.]

TP: Group assignemtns (Exercise form each TP) [5 val.]

 

Final exam [20 val.]

 

Grade improvement (or any extraordinary situations) will be resolved by oral examination.

Subject matter

1. Historical aspects: from Mendelian heredity to genetic engineering

2. Fundamentals of DNA chemistry: Eukaryote vs. prokaryote chromosomes

3. Nuclear division in eukaryotes

4. Fundamentals of genetics and heredity

5. Mechanisms of gene expression in eukaryotes

6. DNA isolation, amplification and sequencing

7. Principles of genetic engineering: heterologous expression in prokaryotes

8. Principles of genetic engineering: the yeast model

9. Principles of genetic engineering: plans and animal cell cultures

10. Genetics modified organisms and gene editing

Programs

Programs where the course is taught: