Instrumental Methods of Analysis I


The main objectives of learning in the Instrumental Methods of Analysis 1 discipline are to make students understand, distinguish and master different classical and modern chemical analysis techniques, such as ultraviolet-visible, fluorescence, infrared and light scattering ( DLS, Raman, SERS).

General characterization





Responsible teacher

Carlos Lodeiro Espino, César Antonio Tonicha Laia


Weekly - 2

Total - 31

Teaching language



It is recommended that the student has passed subjects such as Introduction to Chemistry-Physics, Analytic Chemistry, Chemistry-Physics and basic knowledge of Inorganic and Organic Chemistry.


Quantitative Chemical Analysis, D. C. Harris, W. H. Freeman; 9th edition (July 15, 2015)

Teaching method

Theoretical-practical classes with theory concepts and problems.

Practical classes with work delivery at the end of each practice or report.

Preparation of an group work on a subject referred by the teacher in Portuguese or English and oral defense in Portuguese or English.

Evaluation method

Theory Grade (NT): average of 2 tests. Small tests during classes may count 15% of NT only if their grade is higher than the tests.

Practice Grade (NP): 70% Laboratories + 30% Seminar

Final Grade: 50%NT+50%NP

The Final Grade must be equal or above 9.5.

Subject matter

1) Error calculation, data processing, statistics applied to analytical data. Calibration techniques.

2) Optical molecular spectroscopies: UV-VIS absorption and emission molecular spectroscopy (absorption, fluorescence and phosphorescence). Diffraction networks, monochromators and diode vectors, analyzers.

3) Vibrational Spectroscopies: IR molecular spectroscopy, FTIR. Interferometers. Raman and SERS.

4) Polarimetry, Light scattering (DLS).


Programs where the course is taught: