Safety and Laboratory Manipulation


After this unit, students should be able to:
1. Understand the meaning of Biosafety and biological agents’ classification according to the different levels of laboratories in the fields of Microbiology and Parasitology.
2. Know how to proceed for the transport of biological samples according to the existing rules for this purpose.
3. Know how to recognize the difference between decontamination, asepsis and sterilization.
4. Identify the different types of biological safety chambers.
5. Know legislation and international policy in the context of laboratory safety. Act correctly in the event of any potential accident that may occur when using equipment and/or reagents within a laboratory.
6. Knowing how to be in the animal experimentation laboratory according to the rules to be used.
7. Solve exercises applied to the execution of laboratory techniques.
8. Know the meaning of 'Quality Control (QC)' - Standardization of laboratory procedures applied to culture media, reagents, glassware and equipment, ISO standards and CE mark; Legislation (US directives).
9. Know how to distinguish internal and external QC.
10. Identify the Reference Laboratories (eg IHMT Leptospirosis Lab, NOVA); and know what the Proficiency Tests are and what they are for.

General characterization





Responsible teacher

Maria Luísa Vieira


Weekly - 6

Total - 26

Teaching language



Attendance of 2/3 of classes is mandatory


Students have access to slides (PPT) classes and materials assigned by the teachers involved in Curricular Unit, through the Moodle platform and the bibliography indicated below:
• Cooper,G., CLS., MHA. (2008). Basic Lessons in Laboratory Quality Control. QC Workbook. Published by Bio-Rad Laboratories, Inc. Quality Systems Division 60pp.
• IHMT, NOVA (2014). Manual de Segurança Biológica. 50pp.
• WHO. (2018). Laboratory Quality Management System. Handbook, 245 pp.
• Laboratory Biosafety and Biosecurity Risk Assessment Technical Guidance Document.
• Yao, K., McKinney, B., Murphy, A., M. T. (ASCP), Rotz, P., Wafula, W., Sendagire, H., Okui S., MPH, Nkengasong, J. N. (2010). Improving Quality Management Systems of Laboratories in Developing Countries. American Journal Clinical Pathology, 134, 401-409. doi: 10.1309/AJCPNBBL53FWUIQJ.

Teaching method

Lectures (L), Theoretical and Practical (TP) and Laboratory Practice (LP), all of these learning are in line with the objetives, allowing the student can frame the knowledge acquired in initials lectures. Also the tutorials encourage an active student interaction with teachers, allowing answer questions and discuss aspects debated in class.

Evaluation method

Students with at least 2/3 frequency held writing a final test (multiple choice test) with 40 issues covering subject taught.

- Rating Scale 0-20.
- Approval with rating equal to or greater than 10.Students with at least 2/3 frequency, are evaluated through active participation in class and final written exam (multiple-choice test).
Rating scale of 0 to 20. Approval is given with a rating greater or equal to 10.

Subject matter

I. Theoretical classes (Lectures)
Biosafety and biological risk.
Decontamination, asepsis and sterilization.
Quality control (QC) in the Labor.
II. Theoretical and Practical
Biosecurity and accidents in laboratory environment: responsibilities and behavior.
The Laboratory and Animal Experimentation - Guided visit to the vivarium of IHMT-NOVA. Discussion of several basic concepts associated with laboratory animal sciences; Legislation and accreditation of facilities, people and projects; 3Rs Principle: Reduce, Reuse and Refine.
Resolution of exercises applied to laboratory practice (solution preparation and culture media; dilutions from stock solutions; reconstitution primers ...).
III. Laboratorial Practice
Laboratory Manipulation - Titration a phage lysate.
Cell culture.
Count of bacteria (with Petroff-Hauser chamber).


Programs where the course is taught: