Biochemical Engineering
Objectives
The main purpose is to stimulate in the students the ability to understand and develop processes where the reaction rate is controlled by biochemical kinetics. The learned concepts will enable the students to develop, design and operate industrial biotechnology based processes.
General characterization
Code
12184
Credits
6.0
Responsible teacher
Maria Filomena Andrade de Freitas
Hours
Weekly - 4
Total - Available soon
Teaching language
Português
Prerequisites
None
Bibliography
Bailey, J.E. and Ollis, D.F. (1986). Biochemical Engineering Fundamentals. McGraw-Hill, New York, USA.
Nielsen, J. and Villadsen, J. (2011). Bioreaction Engineering Principles. Plenum Press. New York, USA (ISBN 978-1-4419-9687-9)
Doran, P.M. (2012) Bioprocess Engineering Principles, Academic press, London (ISBN: 9780122208515)
Teaching method
Theoretical classes, problem resolution classes and one experimental work.
Visit to a biotechnology based process
Evaluation method
Approval on EB for continuous evaluation requires the following 3 criteria:
1. The arithmetic mean of the 2 midterm examinations has to be higher or equal to 9.5
2. Lab work, report editing and discussion
3. The final mark is weighted (60% mean of 2 midterm examinations + 40% lab work) and has to be higher or equal to 9.5
Approval on EB for final exam requires the following criteria
1. The exam mark has to be higher or equal to 9.5
2. Lab work, report editing and discussion
3. The final mark is weighted (60% exam + 40% lab work) has to be higher or equal to 9.5
Note: Graphic calculators are not permitted during tests or exams
Subject matter
I. Bioreactor analysis and operation
1.1. Determination of stoichiometric and kinetic parameters.
1.1.1. Stoichiometry coefficients for cell growth and product formation (elemental and electron balances, biomass yield and oxygen demand)
1.1.2. Microbial growth kinetics
1.1.3. Product formation and substrate consumption
1.2. Batch reactor, stirred tank reactor (CSTR), fed-batch and plug flow (PFR)
1.2.1. Material balances
1.2.2. Steady state reactor operation
1.2.3. Design and optimization of reactor operation
II- Mass transfer in aerated bioreactors
2.1. Principles of gas-liquid mass transfer
2.2. Material balances in aerated bioreactors
2.3. Type of aerated bioreactors
2.3.1. Bubble columns
2.3.2. Mechanical agitated bioreactors
2.4. Determination of hold up and interfacial area
2.5. Experimental determination of gas-liquid transfer coefficient; use of correlations.
III- Heat transfer in bioreactors
3.1. Heat balances and determination of heat transfer coefficient
3.2. Media sterilization. Batch and continuous sterilization