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Enzyme Technology

Objectives

The students should acquire knowledge to understand the processes of selection, production and purification of a biocatalyst as well as its application and modelling in bioreactors, namely through immobilization techniques.

General characterization

Code

10688

Credits

6.0

Responsible teacher

Ana Cecília Afonso Roque, Maria Filomena Andrade de Freitas

Hours

Weekly - 4

Total - 56

Teaching language

Português

Prerequisites

none

Bibliography

[1] Klaus Buchholz, Volker Kasche, Uwe Theo Bornscheuer (2005), “Biocatalysts and Enzyme Technology”, Wiley-VCH.

[2] Gama, M, Aires-Barros, MR, Cabral, J (2003), "Engenharia Enzinmática", Lidel, ISBN 9789727572724 | 272 págs.

Teaching method

Available soon

Evaluation method

Available soon

Subject matter

(i) Enzyme structure and function.

(ii) Activity and stability of enzymes.

(iii) Enzyme production and purification.Sources of biocatalysts. Methods for enzyme recovery: solid-liquid separation, cell lysis and disruption, protein precipitation, aqueous two-phase liquid extraction, chromatography.

(iv) Protein engineering, molecular modelling. Immobilization of biocatalysts.

(v) Characterization of immobilised biocatalysts. Effects of immobilization on enzyme kinetics, internal and external diffusional limitations.

(vi) Reactors with biocatalysts (soluble and immobilized biocatalysts), project and analysis. Continuous stirred tank reactor, plug flow reactor, batch reactor.