Analytical Bioorganic
Objectives
The main objectives are:
1) learning the application of different chromatographic techniques;
2) learning the sample preparation techniques;
3) understanding the qualitative and quantitative analytical chemistry.
General characterization
Code
11265
Credits
6.0
Responsible teacher
João Paulo da Costa Noronha, Luísa Maria da Silva Pinto Ferreira
Hours
Weekly - 3
Total - 56
Teaching language
Português
Prerequisites
Basic knowledge in Chemistry and Organic Chemistry.
Basic knowledge of statistics.
Bibliography
The Troubleshooting and Maintenance Guide for Gas Chromatographers, Dean Rood, 4th Ed., Wiley-VCH, Weinheim, 2007.
Interpretation of Mass Spectra, McLafferty, F.W. and Turecek, F., University Science Books, Mill Valley, CA, 4th Edition, 1993.
Introduction to Protein Mass Spectrometry, Pradip K. Ghosh, Academic Press 2016, https://doi.org/10.1016/C2014-0-02130-2, ISBN: 978-0-12-802102-6
Organic Laboratory Techniques, Ralph J. Fessenden, Joan S. Fessenden and Patty Feist, Brooks/Cole (Ed.), 2001.
Principles of Analytical Chemistry – A Textbook, M. Valcárcel, Springer-Verlag (Ed.), 2000.
Quantitative Chemical Analysis, 8th Edition - Daniel C. Harris - W. H. Freeman (2010)
Statistics for Analytical Chemistry; J.C. Miller and J.N. Miller, Ellis Horwood Limited, Chichester, England, 1988.
Teaching method
Theoretical subjects are presented and explained to students in theoretical-practical lectures (1h / week).
- The theoretical-practical lectures taught using a "data-show", accompanied by supplementary bibliography previously available on the course page in CLIP/Moodle. In the first class of the semester all information about the curricular unit will be presented. Evaluation will be discussed as well.
Practical lectures, 2.5h/week in a working group. Of the 42 practical hours, 4 hours include seminars and 10 hours are for the student''s personal projects or to safeguard equipment from malfunctioning.
It will be involved: GC, HPLC (LC-UV, LC-DAD), sample preparation methods (including classic methods, SPE and SPME). Introduction to multidimensional methods (GC-MS, LC-MS/MS)
Evaluation method
1.Theoretical-practical assessement:
- 2 theoretical-practical tests (individual): 25% each
2.Practical assessement:
- 1 Report (article format) + Presentation (group) - Overall assessment: 25%
- 2 Delivery of work in Note format (group): 25%
In all assessement periods, marks are rounded to the nearest tenth of a unit. If the average grade for the theoretical-practical part is less than 9.50 out of 20, the grade for the theoretical-practical component will have to be obtained through an exam, which will then be worth 50% of the final grade for the course.
Attendance is compulsory. It is based on 67% attendance at theoretical-practical classes and attendance at all practical classes.
When carrying out the evaluation elements (Theoretical-practical Tests/Examination) it is permitted to use a scientific or graphical calculator as well as a form constructed by the student.
The elements of the laboratory assessment component (Note/Report) are sent to the course moodle page on a date/time agreed with the students.
Subject matter
Instrumentation: HPLC, GC, GC/MS and LC-MS/MS - basic components and how to use.
Sample preparation: SPE, SPME, liquid extraction, lyophilization, dryness and derivatization.
Analytical method validation.