Advanced Methods in Molecular Biology
Objectives
At the end of this course unit students should be able to:
1. Know the structure and function of nucleic acids and proteins.
2. Understand the basic principles of protein and nucleic acid analysis.
3. Understand the principles of monoclonal antibody production and identify the advantages and limitations of their use.
4. Understand the principles of PCR and RT-PCR technologies and identify their advantages and limitations.
5. Understand the fundamental principles of Sanger sequencing
6. Understand the fundamental principles of the different types of next-generation sequencing and identify the advantages and limitations of each of them and their use.
7. Understand the basic principles behind mass spectrometry technology, its advantages and limitations in the identification of peptides and proteins.
General characterization
Code
9533049
Credits
Available soon
Responsible teacher
Available soon
Hours
Weekly - Available soon
Total - 112
Teaching language
PT
Prerequisites
Available soon
Bibliography
Available soon
Teaching method
Theoretical lectures using audiovisual aids and internet resources.
Evaluation method
Assessment by multiple-choice written exam (50%) and assessment of expository and argumentative skills in seminars (50%).
Subject matter
I. Structure and function of proteins and nucleic acids
II. Monoclonal antibodies: production, characterization, humanization and use in research, diagnosis and clinical practice
III. Nucleic acid hybridization
IV. PCR and RT-PCR
V. Sanger sequencing and next generation sequencing (NGS) in the diagnosis, investigation and identification of pathogens
VI. Mass spectrometry.