Glicobiologia e Doença

Objetivos

No final da unidade curricular o aluno deverá ser capaz de:
- Associar a estrutura e função biológica dos glicanos e as proteínas que os reconhecem (lectinas, adesinas);
- Interpretar a estrutura dos glicanos de acordo com a sua classe e via biossintética:
- Determinar as diferentes formas como a glicosilação molda a actividade dos receptores imunitários e tem impacto na resposta imunitária;
- Classificar as proteínas que reconhecem os glicanos de acordo com a sua especificidade e função biológica;
- Nomear as patologias cujos mecanismos envolvem os glicanos;
- Indicar metodologias avançadas em glicobiologia para o estudo de glicanos;
- Explicar os princípios básicos das metodologias/tecnologias utilizadas para analisar a estrutura das latas de glicose;
- Interpretar os resultados de testes diagnósticos simples utilizados na prática clínica para doenças congénitas de glicosilação (CDG) e cancro;
- Explorar a relação entre as estruturas e os mecanismos fisiopatológicos por detrás de certas condições patológicas (por exemplo, cancro e CDG);
- Enumerar medicamentos baseados em glicanos ou em glicoconjugados ou em proteínas que os reconheçam e modelem;
- Formular questões relacionadas com a diversidade, função e reconhecimento dos glicanos e a sua relevância em patologias como a CDG, a infecção e o cancro
- Interpretar publicações científicas e ser capaz de destacar uma matéria no campo da Glicociência;

Caracterização geral

Código

12032

Créditos

3.0

Professor responsável

Maria Angelina de Sá Palma, Paula Alexandra Quintela Videira

Horas

Semanais - 2

Totais - 28

Idioma de ensino

Português

Pré-requisitos

Conhecimentos básicos de Biologia Celular e Molecular, Microbiologia, Bioquímica e Genética Molecular

Bibliografia

Essentials of Glycobiology –Varki A, Cummings RD, Esko JD, et al., editors. Essentials of Glycobiology. 4th edition. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2022-. Available free from: https://www.ncbi.nlm.nih.gov/books/NBK579918/ .

Introduction to Glycobiology –Third Edition Maureen E. Taylor and Kurt Drickamer, Oxford University Press, Inc., New York. 2011, ISBN 978-0-19-956911-3. Available to request in the library as a hard-copy.

Artigos científicos selecionados

Disponibilizados pelos docentes na página MOODLE da disciplina.

Método de ensino

A disponibilizar brevemente

Método de avaliação

Evaluation:
Attendance at theoretical classes is recommended and there is compulsory attendance at least two thirds of practical and theoretical-practical classes.
Grades are given based on these three parameters:
1) A written test with a weighting of 50 %, which includes material from the theoretical and practical classes. This test must have a grade higher than 9/20;
2) Presentation and discussion of an highlight on a glycan or glycan-binding protein with relevance to disease with a weighting of 30%;
3) Class participation, including tasks performed during practical classes, and tasks posed on the moodle platform with a weighting of 20%;
The final mark is given by the sum of the test mark, the mark of the presentation and discussion of the scientific highlight and the mark of the participation. This sum should be higher than 9.5 points to pass the course.

Conteúdo

Day	Type of class/ Syllabus	Teacher
12/09	Theory: Introduction to the curricular unit/ Introduction to Glycobiology. Practical: Introduction to the practical component and bioinformatics tools/platforms made available in the unit - Formation of groups.	Paula Angelina Filipa
19/09	Theory: Introduction to Glycobiology: basic concepts of the structural diversity of glycans Practical: Introduction to glycan identification and structure - exercises to build glycan molecules and use of an international resource for glycans (GlyGen).	Angelina
26/09	Theory: Glycan biosynthesis - N- and O-glycosylation - ER-GOLGI secretion pathway and glycoprotein processing. Practical: Continuation of the exercises on identifying glycans and glycan structure - N- and O-glycans and their function in the cell.	Angelina
3/10	Theory: Glycan Recognition - Lectins and Antibodies Practical: Visit to the GlycoLab - Microarray facility. Virtual exhibition of a microarray laboratory, specialised equipment and methodologies	Angelina
10/10	Theory: "Glycan-mediated microbial adhesion" - e-learning session (moodle interface only). Practical: Moodle Quizzes	Paula
17/10	Theory: Advanced methodologies in Glycobiology for the study of glycans - from the molecule to the cell Practical: Analysis of glycan microarray data - identifying fine specificity for glycans and discovering new ligands in infection	Angelina
24/10	Theory: Glycobiology and Inflammation: Recognition of glycans by the immune system and immunomodulation. Inflammatory process and cell migration. Practical: Flow cytometry - exercises (lectin staining)	Paula
31/10	Theory: Molecular recognition of glycans by lectins in disease. From structure to function. Practical: Visit to NMR laboratory	Filipa
7/11	Theory: Glycans in cancer: biomarkers and treatment opportunities. Practical: Analysis of tissue samples by immunohistochemistry and discussion.	Paula
14/11	Theory: Congenital disorders of glycosylation (CDGs) and human therapy Practical: Clinical case discussion: from mutation to diagnosis/treatment	Paula
21/11	Theoretical-practical classes: Presentation and discussion of a scientific highlight on Glycobiology	Paula, Angelina, Filipa
28/11	Glycan-based therapies	Paula
13/12	Test	Paula Angelina Filipa

Cursos

Cursos onde a unidade curricular é leccionada: